Figure 6.

Monitoring G1-phase arrest by palbociclib using CycE-Luc2 reporter in MCF-7 breast cancer cells. MCF-7 cells were transiently transfected with CycE-Luc2 reporter. Later, cells were treated with palbociclib (Pal, 1 µM) for 24 or 48 h. Cells lysates were used for analyzing the expression of CycE-Luc2 fusion protein by immunoblotting (A). The cell content (B) and cycle distribution (C) were analyzed by flow cytometer. The luciferase activity of CycE-Luc2 was measured by bioluminescence imaging (D). (E) Quantitative analyses of average luminescence intensities of D. All the groups have four replicates and the experiments were repeated for three times. Error bars indicate standard error. ***: p<0.001.