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. 2021 Feb 3;17(3):756–767. doi: 10.7150/ijbs.52927

Figure 4.

Figure 4

AKR1B10 activates the NF-κB pathway. (A) The levels of IKBα/NF-κB signaling pathway protein at CNE-2/AKR1B10 cells were assessed by Western blot after IR treatment (0 and 2 Gy) at 4, 12 and 24 h respectively. (B and C) The quantificated statistics results of phosphorylated NF-κB and phosphorylated IKBα protein expression relative to β-actin protein level. The experiments were repeated three times. (D) Construction of AKR1B10-knockdown cell line. (E and F) Knockdown of AKR1B10 downregulates the phosphorylation level of NF-κB. IR: irradiation; Gy: GrayA; A: AKR1B10; V: vector, psin-EF2-puromycine; CNE-2/A: CNE-2/AKR1B10, AKR1B10 expressed CNE-2 cells; CNE-2/V: CNE-2/AKR1B10 vector, CNE-2 control cells; *P < 0.05; **P < 0.01.