Skip to main content
. 2021 Feb 8;17(3):796–806. doi: 10.7150/ijbs.54091

Figure 4.

Figure 4

Effects of miR-1290 regulated by NONHSAT024778 on chordoma cells proliferation, migration and invasion in vitro (*P<0.05). (A, B) qRT-PCR was used to measure mRNA level. Left, The NONHSAT024778 mRNA level of si-NONHSAT024778+miR-1290 inhibitor or Lv-NONHSAT024778+miR-1290 mimic transfected U-CH1 cell compared with control groups. Right, the miR-1290 mRNA level of si-NONHSAT024778+miR-1290 inhibitor or Lv-NONHSAT024778+miR-1290 mimic transfected U-CH1 cell compared with control groups. (C, D) Growth curves of CCK8 assays for U-CH1 cells after transfected with si-NONHSAT024778+miR-1290 inhibitor or Lv-NONHSAT024778+miR-1290 mimic compared with control groups. (E, F) Invasion ability of U-CH1 cells after transfected with si-NONHSAT024778+miR-1290 inhibitor or Lv-NONHSAT024778+miR-1290 mimic or control vector was determined by Transwell assay. (G, H) Migration ability of U-CH1 cells after transfected with si-NONHSAT024778+miR-1290 inhibitor or Lv-NONHSAT024778+miR-1290 mimic or control vector was determined by wound healing assay. (I, J) The mRNA and protein expression of Robo1 was measured in U-CH1 cells transfected with si-NONHSAT024778+miR-1290 inhibitor or Lv-NONHSAT024778+miR-1290 mimic or control vector.