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. 2021 Feb 8;17(3):796–806. doi: 10.7150/ijbs.54091

Figure 5.

Figure 5

Robo1 is a target of miR-1290 and is regulated by NONHSAT024778/miR-1290(*P<0.05). (A) Bioinformatic analysis identified a potential miR-1290 target site in the 3'UTR of Robo1. (B) A photograph displays the luciferase reporter plasmid containing wild type (WT) or mutant (Mut) Robo1. (C) The luciferase reporter plasmid Robo1-3'UTR-WT or Robo1-3'UTR-Mut was co-transfected into cells with miR-1290 in parallel with an empty plasmid vector. (D) Relative mRNA level of Robo1 in U-CH1 cells transfected with si-NONHSAT024778+miR-1290 inhibitor, si-NC+miR-1290 inhibitor, si-NONHSAT024778 or scramble. (E) Relative mRNA level of Robo1 in U-CH1 cells transfected with Lv-NONHSAT024778+miR-1290 mimic, Lv-NC+miR-1290 mimic, Lv-NONHSAT024778 or Lv-NC.