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. 2021 Feb 8;17(3):834–847. doi: 10.7150/ijbs.57099

Figure 3.

Figure 3

AGR2 affects fatty acid uptake and utilization. A. Hierarchical clustering heatmaps of differentially expressed proteins between the livers of WT and Agr2-/- mice. B. Results of GO term analysis of differentially expressed proteins between the livers of WT and Agr2-/- mice. C. Statistical results of analysis of the KEGG pathways associated with the differentially expressed proteins between the livers of WT and Agr2-/- mice. D. Lipid metabolism gene expression in the livers of the 8-week-old WT and Agr2-/- mice fed a NCD (n=3). E. Analysis of FASN activity in the cytoplasm in the livers of the 8-week-old WT and Agr2-/- mice fed a NCD (n=5). F. Western blot analysis of lipid metabolism protein levels in the livers of the 8-week-old WT and Agr2-/- mice fed a NCD. Quantification was performed by normalizing proteins to β-actin. G. Immunohistochemical staining of FABP1, ACSL3 and ACSL5 in the livers of the 8-week-old WT and Agr2-/- mice fed a NCD. H. Western blot analysis of FABP1, ACSL3 and ACSL5 in the livers of the 18-week-old WT and Agr2-/- mice fed a NCD. Quantification was performed by normalizing proteins to β-actin. I. Immunohistochemical staining of FABP1, ACSL3 and ACSL5 in the livers of the 18-week-old WT and Agr2-/- mice fed a NCD. J. Western blot analysis of FABP1, ACSL3 and ACSL5 in the livers of 8-week-old WT and Agr2/Tg mice fed a HFD for an additional 10 weeks. Quantification was performed by normalizing proteins to β-actin. K. Immunohistochemical staining of FABP1, ACSL3 and ACSL5 in the livers of the 8-week-old WT and Agr2/Tg mice fed a HFD for an additional 10 weeks. L. Flow cytometric analysis of lipid absorption in primary hepatocytes from WT and Agr2-/- mice. M. Flow cytometric analysis of lipid absorption in primary hepatocytes from WT and Agr2/Tg mice. N. Analysis of the ketone body content in the cytoplasm in the livers of 8-week-old WT and Agr2-/- mice fed a NCD or a HFD for an additional 10 weeks (n=5). Representative figures were generated with data from at least three independent experiments. The data are presented as the mean ± SD values. *P < 0.05 by Student's t test.