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. 2021 Feb 8;17(3):834–847. doi: 10.7150/ijbs.57099

Figure 4.

Figure 4

AGR2-mediated effect on fatty acid metabolism depends on FABP1. A. Western blot analysis of lipid metabolism protein levels in whole-cell lysates from Huh7 cells treated with siRNA targeting AGR2. Western blot analysis of lipid metabolism protein levels in whole-cell lysates from HepG2 cells treated with AGR2 and AGR2-C81A expression plasmids. Quantification was performed by normalizing proteins to β-actin. B. TG in primary hepatocytes from WT and Agr2/Tg mice treated with siRNA targeting FABP2. C. TG in primary hepatocytes from WT and Agr2/Tg mice treated with siRNA targeting ACSL3. D. TG in primary hepatocytes from WT and Agr2/Tg mice treated with siRNA targeting ACSL5. E. TG and lipid droplet staining in primary hepatocytes from WT mice treated with siRNA targeting FABP1. F. TG and lipid droplet staining in primary hepatocytes from WT and Agr2/Tg mice treated with siRNA targeting FABP1. G. TG and lipid droplet staining in primary hepatocytes from WT and Agr2-/- mice treated with siRNA targeting FABP1. H. TG content in HepG2 cells treated with AGR2 and AGR2-C81A expression plasmids and siRNA targeting FABP1. Lipid staining in HepG2 cells treated with AGR2 and AGR2-C81A expression plasmids and siRNA targeting FABP1. Representative figures were generated with data from at least three independent experiments. The data are presented as the mean ± SD values. *P < 0.05, **P < 0.01, ***P < 0.001 by Student's t test.