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. 2021 Mar 19;8(2):rbab013. doi: 10.1093/rb/rbab013

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© The Author(s) 2021. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — In vitro cytocompatibility evaluation of smooth and porous microcarriers. (a) Cell viability was evaluated by culturing MC3T3-E1 cells in smooth and porous microcarriers extracts for 24 h through CCK-8 assay kit. (b) Fluorescent and SEM micrographs of MC3T3-E1 cells on smooth and porous microcarriers at 1, 3 and 7 days post seeding. The cells were stained with Calcein AM (cytoplasm, green) and DAPI (nucleus, blue). The scale bar lengths are 200 μm and 10 μm, respectively. (c) Proliferation of MC3T3-E1 cells on smooth and porous microcarriers at 1, 3 and 7 days post seeding were evaluated by CCK-8 assay kit (*P ≤ 0.05, n = 3 for each group)