KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-PD-L1 (clone 10F.9G2) | Biolegend | Cat No. 124313 |
| Anti-MHC class I H-2Db (clone KH95) | Biolegend | Cat No.111518 |
| Anti-MHC class I H-2Kb (clone AF6–88.5) | BD | Cat No. 561072 |
| Anti-mouse PD-1 (clone RMP1.14) | BioXcell | Cat No. BE0146 |
| Anti-mouse PD-L1 (clone 10F.9G2) | BioXcell | Cat No. BE0101 |
| Primary antibody against mouse mesothelin (clone B35) | MBL | Cat No. D233–3 |
| Goat anti-rat secondary antibody conjugated to AF488 | Thermo Fisher | Cat No. A-11006 |
| Agonistic anti-mouse anti-CD40 (clone FGK45) | BioXcell | BE0016–2 |
| Fc block (anti-CD16/32) | Tonbo | 70-1061-U100 |
| Anti-CD45 (clone 30-F11) | Biolegend | 103147 |
| Anti-CD8a (clone 53–6.7) | Thermo Fisher | 45-0081-82 |
| Anti-CD44 (clone IM7) | BD | 564392 |
| Anti-CD62L (clone MEL-14) | Biolegend | 104406 |
| Anti-PD-1 (CD274, clone J43) | Thermo Fisher | 25-9985-82 |
| Anti-KLRG1 (clone 2F1) | Biolegend | 138408 |
| Anti-Tim-3 (clone RMT3-23) | Biolegend | 119725 |
| Anti-Lag-3 (CD223, clone C9B7W) | Biolegend | 125227 |
| Anti-CD19 (clone 1D3) | BD | 563557 |
| Anti-F4/80 (clone T45-2342) | BD | 565614 |
| Anti-CD11c (clone N418) | BD | 744180 |
| Ghost dye live-dead in BV510 | Tonbo | 13-0870-T500 |
| Ghost dye live-dead in APCef780 | Tonbo | 13-0865-T500 |
| Anti-IFN-gamma (clone XMG1.2) | Biolegend | 505810 |
| Anti-TIGIT (clone 1G9) | Biolegend | 372706 |
| Anti-CD69 (clone H1.2F3) | Biolegend | 104506 |
| Anti-Ki67 (clone 16A8) | Biolegend | 652418 |
| Goat anti-mouse PD-L1 | R&D systems | AF1019-SP |
| Rat anti-mouse CD31 | BD | 550274 |
| Rabbit anti-mouse Lyve-1 | Novus | NB600–1008 |
| Anti-panCK-FITC (clone C-11) | Sigma-Aldrich | F3418 |
| Anti-goat AF647 | Jackson Immunoresearch | 705-605-147 |
| Anti-rat AF546 | Invitrogen | A-11081 |
| Anti-rabbit AF647 | Life Technologies | A27040 |
| Anti-F4/80 (clone BM8) | Tonbo | 4801-U100 |
| Anti-αSMA (clone 1A4) | Thermo FIsher | 14-9760-82 |
| Rabbit anti-mouse Ly6G | BD | 551459 |
| Anti-LFA-1 (clone H155–78) | Biolegend | 141008 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| FTY720 | Cayman Chemical | Cat No. 10008639 |
| D-Luciferin | Gold Bio | LUCK-100 |
| Poly I:C | Sigma-Aldrich | P1530 |
| Recombinant mouse IFNγ | R&D systems | 485-MI-100 |
| Click beetle red luciferase derived peptides | Genscript | N/A |
| Streptavidin R-PE | Invitrogen | S866 |
| Red blood cell lysis buffer | GIBCO | A1049201 |
| DAPI Prolong Gold | Life Technologies | P36931 |
| Amphotericin B | GIBCO | 15290018 |
| Penicillin/streptomycin | GIBCO | 10378016 |
| Dextrose | Fisher Scientific | D15–500 |
| L-glutamine | Thermo Fisher | 25030081 |
| Blasticidin | Thermo Fisher | A1113903 |
| Puromycin | Thermo Fisher | A1113803 |
| EDTA | Thermo Fisher | 15575020 |
| Collagenase IV | Sigma Aldrich | V900893 |
| NEAA | GIBCO | 11-140-050 |
| Betamercaptoethanol | GIBCO | M6250 |
| D-PBS (no phenol red) | GIBCO | 14040133 |
| Golgiplug | BD | 555029 |
| OCT Tissue-Tek | Electron Microscopy Sciences | 6255001 |
| Acetone | Sigma Aldrich | V800023 |
| Critical Commercial Assays | ||
| BD fixation kit | BD | 554714 |
| Foxp3 fixation and permeabilization kit | Tonbo | TNB-0607-KIT |
| Effectene kit | QIAGEN | 301425 |
| QIAgen RNeasy kit | QIAGEN | 74104 |
| SYBR Green master mix | Thermo Fisher | 4344463 |
| High Capacity RNA to cDNA kit | Thermo Fisher | 438706 |
| Experimental Models: Cell Lines | ||
| KPC PDA cells and clones | This paper | N/A |
| Plat-E cells | CELL BIOLABS | RV-101 |
| Experimental Models: Organisms/Strains | ||
| C57BL/6J KPC mice | Fred Hutchinson Cancer Research Center | Stromnes et al., 2015 |
| C57BL/6J mice | Jackson laboratories | 000664 |
| Oligonucleotides (primers) | ||
| Msln R 5’ TGG ACC TTG TGA ACG AGA TTC 3’ | This paper | IDT |
| Msln F 5’ TGG ATC AGG GAC TCA GGA TAG 3’ | This paper | IDT |
| H2-Db R 5’ GGT GAC TTC ACC TTT AGA TCT GGG 3’ | Lv, D. et. al., 2015 | IDT |
| H2-Db F 5’ AGT GGT GCT GCA GAG CAT TAC AA 3’ | Lv, D. et. al., 2015 | IDT |
| H2-Kb R 5’ GGT GAC TTT ATC TTC AGG TCT GCT 3’ | This paper | IDT |
| H2-Kb F 5’ GCT GGT GAA GCA GAG AGA CTC AG 3’ | This paper | IDT |
| Tap1 R 5’ AGT TCA GGC AGA AGT TGG AAG 3’ | This paper | IDT |
| Tap1 F 5’ GCC CAG GTA CAG AAT TCC C 3’ | This paper | IDT |
| Lmp2 R 5’ GGT TAT GTG GAC GCA GCT TA 3’ | This paper | IDT |
| Lmp2 F 5’ GGT GAC CAG GTA GAT GAC AC 3’ | This paper | IDT |
| Lmp7 R 5’ GCT GCT TTC CAA CAT GAT GC 3’ | This paper | IDT |
| Lmp7 F 5’ CCG AGT CCC ATT GTC ATC TAC 3’ | This paper | IDT |
| ATP5b R 5’ CAA TGC AGG AAA GGA TCA CCA 3’ | This paper | IDT |
| ATP5b F 5’ CAT CCA AAT GGG CAA AGG TG 3’ | This paper | IDT |
| Recombinant DNA | ||
| CBR-GFP retroviral vector | Stromnes et al., 2015; Smith et al., 2017 | N/A |
| GFP-only retroviral vector | This report | N/A |
| Ovalbumin-GFP retroviral vector | This report | N/A |
| Software and Algorithms | ||
| NetMHC version 4.0 | http://cbs.dtu.dk/services/NetMHC/ | N/A |
| SYFPEITHI | http://www.syfpeithi.de/bin/MHCServer.dll/EdiptopePrediction.htm | N/A |
| BIMAS | https://bimas.cit.nih/gov/ | N/A |
| FlowJo version 10 | FlowJo | N/A |
| FACS Diva | BD | N/A |
| GraphPad | Prism | N/A |
| IVIS100 with Living Image Software | Xenogen | N/A |
| Imaris9.1.0 | Bitplane | N/A |
| Vevo 2100 | Visual Sonics Fujifilm | N/A |
| Real Touch CFX96 PCR detection system | BioRad | N/A |
| Other | ||
| DMEM | GIBCO | 11960051 |
| FCS | GIBCO | 26140079 |
| Cell counting beads | Sigma | P7458 |
| Magnetic beads conjugated to PE | Miltenyi | 130-048-801 |
| Matrigel | Discovery Labware | 08-774-552 |
| 4.0 Sutures | Ethicon | 1611G |
| 5.0 Sutures | Ethicon | 8580H |
| Magnetic beads conjugated to APC | Miltenyi | 130-090-855 |
| LS Columns | Miltenyi | 130-042-401 |
| QuadroMACS separator | Miltenyi | 130-090-076 |
| CB101–109-H-2Db tetramer | This report | N/A |
| SIINFEKL-H-2K-b tetramer - APC | http://tetramer.yerkes.emory.edu/reagents/class-I-mhc | N/A |