Figure 3.

XIST directly targets miR-155-5p and downregulates its expression. (A-C) Relative mRNA expression of miR-155-5p was determined using RT-qPCR. (D) Relative mRNA expression of XIST was determined using RT-qPCR. (E) The binding sites between XIST and miR-155-5p. (F) The relative luciferase activities in the human cardiac fibroblast cells were evaluated using a luciferase reporter assay. The data are presented as the mean ± standard error of the mean, from three independent experiments. ^*P<0.05, ^**P<0.01, ^***P<0.001 vs. control. miR, microRNA; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; WT, wild-type; MUT, mutant; NC, negative control; Ang II, angiotensin II; sh, short hairpin.