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. 2021 Feb 4;18(7):1609–1617. doi: 10.7150/ijms.52206

Figure 1.

Figure 1

RA suppressed tumor growth, migration, invasion and inhibited the expression of β-catenin and EMT-related proteins in GBM. (A) The U87, U251, T98G and LN299 cell lines were treated with different concentrations of RA for 48 hours and then cell viability was determined using CCK-8 assay. (B) U87 and U251 cells were incubated for 48 hours with the indicated doses of RA and then EdU assay was performed. (C, D) U87 and U251 cells were treated with the indicated doses of RA for 24 hours, and then transwell assay was applied to determine the levels of migration and invasion. (E) qPCR and (F) western blotting analysis were performed on U87 and U251 cells treated with different doses of RA for 48 hours to analyze the mRNA and protein expression levels of β-catenin and EMT-induced proteins. n=3 or 4 and all tests were performed in triplicate. *, P < 0.05 compared with the control (0 μM).