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. 2021 Feb 4;18(7):1609–1617. doi: 10.7150/ijms.52206

Figure 2.

Figure 2

Overexpression of β-catenin reversed the suppression of cell proliferation, migration, invasion and EMT induced by RA. (A, B) U87 cells and U251 cells transfected with β-catenin or the vector plasmid for 18 hours were seeded into 96-well plates and incubated with 100 μM RA or the vehicle for 24 hours. CCK-8 assay was performed to determine the level of cell proliferation. (C, D) U87 cells and U251 cells were transfected with β-catenin or the vector plasmid and were seeded into a transwell chamber and examined using migration and invasion assays. (E) U87 cells and U251 cells transfected with β-catenin or the vector plasmid were then incubated with 100 μM RA for 48 hours. Western blotting analysis was performed to detect protein levels. *, P < 0.05 vs control; #, P < 0.05 compared with either RA treatment or β-catenin transfection alone.