Figure 5.

AFC promoted differential gene expression profiles of proliferation, autophagy, and ferroptosis in NSCLC cell lines. (A) A protein-protein interaction network prediction software result showing that GPX4 catalysed the expression of downstream GSS/GSR-GGT axis. (B) GO analysis results showing that the expression level of GPX4 significantly affected biological processes, such as small molecule metabolic processes, as well as molecular functions, such as catalytic activity. (C) KEGG pathway analysis results showing that GPX4 was highly correlated with the regulation of glutathione metabolism and ferroptosis signalling pathways. (D) Western blotting results showing that AFC significantly affected the expression of the GPX4-GSS/GSR-GGT axis, autophagy-associated proteins, and ferroptosis regulatory proteins in NSCLC cell lines.