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. 2021 Feb 24;18(8):1848–1856. doi: 10.7150/ijms.55866

Figure 3.

Figure 3

The effects of BME on Akt and AMPK. A, B, and C, western blot analysis of the levels of phosphorylated Akt, total Akt (A, B), and phosphorylated AMPK, total AMPK (C). IEC-18 cells were pretreated with 20 ng/mL TNF-α, followed by stimulation with 100 or 200 μg/mL BME, or 50 μM rosiglitazone (R) in the presence or absence of 100 nM insulin, as indicated underneath the blots. In C, the concentration of BME (indicated as B) was 200 μg/mL. Experiments were performed three times independently. The result from one of the experiments is shown. D, glucose uptake assays in IEC-18 cells. Cells were pretreated with 20 ng/mL TNF-α, followed by the treatment as indicated underneath the histogram. Experiments were performed in triplicate. The data are the mean ± SE (N = 3). *P < 0.05 and **P < 0.005 between the indicated groups.