BME promoted the secretion of GLP-1 from NCI-H716 cells. A, cytotoxicity assays. NCI-H716 cells were treated with the solvent (0; control), 10, 50, 100 or 200 μg/mL BME for 6 h and subjected to a cell proliferation assay. Experiments were performed twice independently, each in triplicate. The data are the mean ± SE (N = 6). B and C, GLP-1 secretion assays. NCI-H716 cells were treated for 1 h with 200 μg/mL BME, 10 μM U73122, 10 μM gallein, 10 mM denatonium benzoate (DB), or the respective solvent, as indicated underneath the histogram. The culture medium was subjected to GLP-1 concentration assay by using an ELISA kit. Experiments were performed in quadruplicate (B) or in triplicate (C). The data are the mean ± SE. *P < 0.05 and **P < 0.005 versus the control (A) or between the indicated groups (B and C).