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BME induced calcium responses in NCI-H716 cells. Cells were treated with 200 μg/mL BME or the solvent, or pretreated with 10 μM gallein or 10 μM U73122 for 30 min before the addition of 200 μg/mL BME, and subjected to calcium fluorescent imaging assays. The arrow indicates when BME or solvent was added to the medium. Experiments were performed three times independently. The result from one of the experiments is shown.
