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. 2021 Feb 18;18(8):1810–1823. doi: 10.7150/ijms.51654

Figure 1.

Figure 1

HSM down-regulates excessive autophagy in BLM-induced pulmonary fibrosis mice. (A) In vivo experimental design: Mice were intratracheally administered with BLM (3 mg/kg in 100 μl saline) on day 0, and HSM (200 mg/kg in 500 μl saline) was orally administered after the BLM administration from day 7 to day 27. On day 28, the mice were sacrificed and the corresponding samples were collected. (B) Transmission electron microscopy image of mouse lung tissue. Black arrow: autophagosome; yellow asterisk: cell nucleus; scale bar: 2 μm. The black bars represent the mean ± SEM values between the treated groups analyzed, n=4. (C) Analysis of the changes of LC3B, P62 and ATG5 proteins in mice of each group by Western blotting, n=3. Data are presented as means ± SEM of at least three separate experiments. *P < 0.05, **P < 0.01.