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. 2021 Feb 18;18(8):1810–1823. doi: 10.7150/ijms.51654

Figure 2.

Figure 2

HSM reduces the number of alveolar macrophages in the fibrotic lung. (A) BALF was centrifuged and coated to count the changes of total cells, macrophages, lymphocytes, eosinophils and neutrophils. The CON, HSM, BLM and BLM+HSM mainly display representative morphological images of macrophages, lymphocytes, eosinophils and neutrophils. Scale: 10 μm, magnification: x200, n=4. (B) Gating strategy mainly used to identify macrophages in BALF and lung tissue of mice. The differential expression of CD11b and CD11c was used to distinguish pulmonary macrophages. (C) Effect of HSM on the number of total cells, CD45+ cells and alveolar macrophages in BALF, n=6. (D) Effect of HSM on the number of total cells, CD45+ cells, alveolar macrophages and interstitial macrophages in lung tissue, n=10. Data are presented as means ± SEM of at least three separate experiments. *P < 0.05, **P < 0.01, ***P < 0.001.