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. 2021 Mar 19;11:179. doi: 10.1038/s41398-021-01287-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Overall schematic of pHrodo-labeled quantitative synaptosome phagocytosis assay by CB-iMGs. b Representative live real-time images in phase contrast/red fluorescence overlay mode showing cellular uptake and c red fluorescence channel alone of pHrodo (red)-labeled synaptosomes uptake at the end of the phagocytosis assay (315 min). Scale bar: 60 μm (boxes show magnified view of engulfing CB-iMG).