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. 2021 Mar 18;20:71. doi: 10.1186/s12934-021-01553-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Time course of transgalactosylation reaction detected by TLC analysis. Homo-condensation reactions using ONP-β-gal as donor and acceptor (a). Hetero-condensation reactions performed with PNP-β-glu (b) and PNP-β-xyl (c) as acceptors and ONP-β-gal as donor. Transgalactosylation reactions using D-lactose as substrate (d). Red, blue and green circles show the UV signals obtained from the arylic group of ONP-β-gal, PNP-β-glu, and PNP-β-xyl respectively. STD1: Standard with ONP-β-gal and D-galactose, STD2: Standard with PNP-β-glu and D-glucose, STD3: Standard with PNP-β-xyl and D-xylose, STD4: Standard with D-glucose, STD5: Standard with D-galactose and D-lactose, S: Samples collected at different times (min), B: Blank at different times, S_D: Sample diluted, B_D: Blank diluted, TP: Transgalactosylation products