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Kinetic parameters of BcGalB
| Substrate | KM (mM) | kcat (s−1) | kcat/KM (mM−1 s−1) |
|---|---|---|---|
|
ONP-β-gal D-lactose |
0.723 136.2 |
5466.7 1603.7 |
756.2 11.8 |
KM, kcat and kcat/KM values towards the natural and artificial substrates are reported. Standard deviations were lower than 2% of the calculated values
