Table 4.
Summary of Med subunits in host defense against pathogens.
| A. fumigatus | E. faecalis | M. robertsii natural infection | C. glabrata | Ecc15 | M. robertsii Injection | Lethality at 29°C | RNAi efficiency by dPCR | |
|---|---|---|---|---|---|---|---|---|
| Med1 | L | – | – | – | – | / | ++ | / |
| Med4 | – | – | – | – | – | / | +/- | + |
| Med6 | + | – | – | – | – | / | ++ | / |
| Med7 | L | – | +? | – | – | / | ++ | / |
| Med8 | + | + | – | – | – | / | ++ | / |
| Med9 | – | – | – | – | – | / | +/- | + |
| Med10 | – | – | – | – | – | / | +/- | + |
| Med11 | + | – | – | – | – | / | ++ | / |
| Med12 | L | + | / | – | – | / | ++ | / |
| Med13 | L | – | – | – | – | / | ++ | / |
| Med14 | + | / | – | – | / | / | ++ | / |
| Med15 | L | + | – | – | – | / | ++ | / |
| Med16 | L | – | – | – | – | / | + | / |
| Med17 | + | + | – | – | – | – | ++ | + |
| Med18 | – | – | – | – | – | / | +/- | +/- |
| Med19 | – | – | – | – | + | / | + | / |
| Med20 | – | – | – | – | – | / | + | + |
| Med21 | -? | – | / | – | / | / | ++ | / |
| Med22 | + | + | – | – | – | / | ++ | / |
| Med23 | – | – | – | – | – | / | ++ | / |
| Med24 | – | – | – | – | – | / | ++ | / |
| Med25 | – | – | – | – | – | / | ++ | / |
| Med26 | – | – | – | – | – | / | ++ | / |
| Med27 | -? | – | + | – | – | / | ++ | / |
| Med28 | L | + | – | – | – | / | ++ | / |
| Med29 | L | – | +? | – | – | / | ++ | / |
| Med30 | + | + | – | + | – | / | ++ | / |
| Med31 | + | + | – | – | – | + | ++ | + |
| Cyc C | – | – | – | – | – | / | + | / |
| CDK8 | – | – | – | – | – | / | +/- | / |
The underlined Med subunits on the left column correspond to subunits homologous to essential subunits in the mammalian Mediator complex. L in the A. fumigatus column indicates that uninfected controls succumbed at the same time rate as challenged flies, making it difficult to draw any conclusion with regards to susceptibility to this challenge. ?: the phenotype is not certain as mock-infected controls displayed some sensitivity to the infection procedure. The column next to the right-most column recapitulates the developmental phenotypes shown in Table 1: ++: no escapers; +: some pupal escapers that did not reach the adult stage; +/-: some adult escapers. The right-most column shows the results of monitoring the steady-state transcripts of the targeted gene; -: efficient RNAi with few transcripts detected; +/-: partial depletion of transcripts. Similar results were obtained by “classical” RTqPCR, which is, however, not as precise. /: not tested. Med subunit in bold: the RNAi line is unlikely to function efficiently.