Table 1.
Primers used for detection of gene mutation
| Target Gene | Primer Sequence | Amplicon Size (bp) | GC% | |
|---|---|---|---|---|
| PIK3CA_1 | F | AGACAATGAATTAAGGGAAAATGAC | 690 | 32% |
| R | GTTATACCACTCTTCATATAGCTCA | 36% | ||
| PIK3CA_2 | F | AGTGGGGTAAAGGGAATCAAAAGA | 533 | 41.7% |
| R | GCAATTCCTATGCAATCGGTCT | 45.5% | ||
| EGFR | F | ACGAAGCCTGTGTGTTTGGT | 588 | 50% |
| R | GGCAAAGGGAGTGGAAGGAA | 55% | ||
| KRAS_1 | F | CTTAAGCGTCGATGGAGGAGT | 545 | 52.4% |
| R | ACCCTGACATACTCCCAAGGA | 52.% | ||
| KRAS_2 | F | TGTCCGTCATCTTTGGAGCA | 549 | 50% |
| R | TTTCAATCCCAGCACCACCA | 50% | ||
(R = Reverse primer, F=Forward primer. Suffix with an underscore after the gene name indicates primer set number)