Fig. 5.

Nox4 inhibition counteracts T2DM-induced endothelial dysfunction partially through the Nrf2–Nox4 loop. a Cell lysates of HUVECs were used to detect indicated protein levels by immunoblotting. b–d The quantitative analysis of each immunoblots. e The mRNA level and the quantification of the Nox4 were evaluated by qRT-PCR. f Cell lysates of HUVECs were used to detect indicated protein levels by immunoblotting. g and h The quantitative analysis of each immunoblots. i The mRNA level and the quantification of the Nox4 were evaluated by qRT-PCR, which assay of HUVECs treated as indicated in (f). j Cell lysates of HUVECs were used to detect indicated protein levels by immunoblotting. k, l and m The quantitative analysis of each immunoblots in (j). n Cell lysates of HUVECs were subjected to immunoblot with Nox4 antibody. o The quantitative analysis of (n). p The mRNA level and the quantification of the Nrf2 target gene were evaluated by qRT-PCR. Data are represented as means ± SEM (n = 5). Significance: *P < 0.05 and **P < 0.01 vs. MAN in scrambled HUVECs, #P < 0.05 and ##P < 0.01 vs. HG-PA in scrambled HUVECs. All above results in graphs were normalized to first group