Figure 11.

Everolimus and auranofin cooperate to inhibit tumor growth in nude mice. (A) Everolimus (5 mg/kg) and auranofin (3 mg/kg) combined treatment significantly decreased tumor volume and tumor weight (B) of HCT116 human colon cancer xenografts in nude mice, but did not significantly affect body weight (C) of mice. (D) HE staining of the major organs. (E) The TrxR activity in tumor tissues. (F) MDA levels in tumor tissues. (G-I) The protein levels of LC3-I, LC3-II, p-JNK, JNK, ATF4 and GAPDH were determined by Western blot analyses. (J) The proposed working model. Scale bar = 75 µm (* p < 0.05, ** p < 0.01).