Figure 5.

NAC pretreatment significantly reversed the combined treatment-induced ER stress. (A-B) Cells were pretreated with NAC (5 mM) for 2 h and cell lysates were blotted with the indicated antibodies after treated with the combination for 6 h. (C-D) Cells were pretreated with NAC (5 mM) for 2 h and CHOP expression was observed by fluorescence microscope after treated with auranofin and rapamycin combination for 12 h. (E-F) Cells were pretreated with NAC (5 mM) for 2 h and CHOP expression was observed by fluorescence microscope after treated with auranofin and everolimus combination for 12 h. Scale bar = 25 µm. Data from three technical replicates.