Figure 3.

Colocalization of exosomes with FITC-Aβ₄₂ in SH-SY5Y cells, exerting neuroprotective effects against Aβ₄₂ oligomers. (A) Exosomes were labeled with PKH26 (red fluorescence), and observed by confocal laser microscopy, scale bar: 10 µm. (B) FACS-based internalization assay. (C, D) Effects of HA-Exo and US-HA-Exo on cell viability in human SH-SY5Y neuroblastoma cells treated with oligomeric Aβ₄₂ or vehicle (DMEM). The cells were preincubated with 10 µM Aβ₄₂ for 24 h prior to the addition of exosomes. The morphological pictures were taken, scale bar: 50 µm. The viability of the cultures was assessed by the CCK-8 assay 72 h after the addition of exosomes. (E) Schematic of exosome production, isolation and its cellular effects.