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. 2021 Feb 15;11(9):4011–4029. doi: 10.7150/thno.50051

Figure 2.

Figure 2

ADSL has oncogenic-like properties in in-vitro and in-vivo models of CRC (A) Immunoblot showing ADSL expression in the DLD-1 cell line at 24, 48 and 72 h post siRNA transfection. (B) Proliferation and (C) migration capacity of DLD-1 ADSL-silenced cells compared to control cells. (D) Immunoblot showing ADSL expression in Caco-2 cells at 24, 48, and 72 h post vector transfection. (E) Proliferation and (F) migration capacity of Caco-2 ADSL-overexpressing cells compared to control cells. (G) Immunoblot showing ADSL expression in DLD-1 cells (upper panel) and Caco-2 cells (lower panel) 72 h post siRNA and/or vector transfection. (H) Rescue experiment showing proliferation capacity of DLD-1 (left) and Caco-2 (right) cells with siRNA and/or vector transfection compared to control cells. (I) Schematic illustration of the CAM assay. (J) Representative pictures and quantification of volume of Caco-2 control (CTR) and ADSL-overexpressed (ADSLox) resected tumors 4 days post-implantation of cells in CAMs (n = 12 tumors from 2 independent experiments); mean ± SD. Values are normalized to the mean of CTR. (K) Representative pictures of Caco-2 tumors extracted 4 days post-implantation. Tissue sections were immunostained with ADSL. Scale bars 20-50 µm. For (C) and (F) 8, 16 and 24 correspond to 32, 40 and 48 h post transfection, respectively.