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. 2021 Feb 25;11(9):4281–4297. doi: 10.7150/thno.51193

Figure 2.

Figure 2

Tumor-homing characteristics of Ze affibody with high affinity for EGFR. A. SDS-PAGE and SEC analysis of purified Ze affibody. B. Binding of Ze affibody to EGFR analyzed by biolayer interfereometry. C. EGFR expression in LS174T and HT29 cells and binding of Ze affibody to these cells. D. Cellular distribution of Ze affibody in LS174T tumor grafts. E. Tissue distribution of Ze affibody in mice bearing LS174T tumor grafts. Organs/tissues derived from mice (N=3) were scanned by an IVIS optical imaging system at 4 h postinjection of CF750-labeled Ze affibody.