Table 1.
Primers used in Q-PCR and ChIP
| Primers | Sequence (5'-3') | |
|---|---|---|
| RORγt (mouse) | Forward | TACCCTACTGAGGACAGG |
| Reverse | CCACATTACACTGCTGGCTG | |
| IL-17A (mouse) | Forward | TACCTCAACCGTTCCACGTC |
| Reverse | TTTCCCAACCGCATTGACACA | |
| IL-21 (mouse) | Forward | TGGATCCTGAACTTCTATCAGC |
| Reverse | CACGAGGTCAATGATGAATGTC | |
| IL-22 (mouse) | Forward | GCAGATAACAACACAGATGTCC |
| Reverse | GTCTTCCAGGGTGAAGTTGAG | |
| SLC1A5 (mouse) | Forward | GTTACCGCCATCACCTCCATCAAC |
| Reverse | GGAAGGCAGCAGACACCAGATTG | |
| SLC38A1 (mouse) | Forward | GAGCACAGGCGACATTCTCATCC |
| Reverse | CATGGCGGCACAGGTGGAAC | |
| GCLC (mouse) | Forward | CTATCTGCCCAATTGTTATGGC |
| Reverse | CCTCCCGTGTTCTATCATCTAC | |
| GCLM (mouse) | Forward | CTTGGAGCATTTACAGCCTTAC |
| Reverse | GTGAGTCAGTAGCTGTATGTCA | |
| GS (mouse) | Forward | CTGATGCTAGAGAGATCTCGTG |
| Reverse | TTCACCCATGTCCAGTGAATAG | |
| il-17 promoter (mouse) | Forward | GCCTTTGTGATTGTTTCTTGCAG |
| Reverse | CCTTGCCCAAAGAAACCCTCTC | |
| il-17 CNS1 (mouse) | Forward | GGAAGGTGCATGTGGCTGACTT |
| Reverse | AATGTGCCAGTCCCTTGGATGA | |
| il-17 CNS2 (mouse) | Forward | GCCTCCCATGTGGTCATTAT |
| Reverse | AGGCTCCTTCTCCATTGGTT | |
| il-17 CNS3 (mouse) | Forward | TTTGCTCATGCCCATATGTC |
| Reverse | TGAACCAACTTTCCCCACTC | |
| il-17 CNS4 (mouse) | Forward | CTCAAATCCGTGTGCCTTCT |
| Reverse | CATCTTGAAGCTGAGGCTGA |