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. 2021 Feb 20;11(9):4232–4250. doi: 10.7150/thno.49819

Figure 6.

Figure 6

A significant increase in the proportion of MDSCs and Foxp3+Treg cells were observed in Sptbn1+/- mice. (A) FACS analysis of MDSCs and CD4+CD25+Foxp3+Treg cells in CD45-gated single cell suspension of liver and peripheral blood was carried out for WT and Sptbn1+/- mice (n = 5, aged 6 to 8 months) after staining with antibodies as indicated. The normalized fold-change (mean ± S.D.) (comparing the % of immune cells to WT group) were shown. Significance of differences was evaluated by Student's t test (*P < 0.05 and **P < 0.01 versus WT group). Representative FACS blots of MDSCs as defined as Gr-1+CD11b+, Gr-1+F4/80+ cells, and Foxp3+Treg cells as defined as CD4+CD25+Foxp3+ cells (within areas indicated by horizontal and vertical lines in red color) from WT and Sptbn1+/- mice liver were shown. (B) Hematoxylin and eosin (HE) and immunohistochemical staining of mouse liver tissues. In liver of Sptbn1+/- mouse (2 of 4 mice, >15months), well differentiated HCC could be observed. The neoplastic cells grow in a thin trabecular pattern. These cells show mild dysplasia with a slight increase of the nucleus to cytoplasm ratio (HE panels). Numbers of Foxp3-positive Treg cells (arrow pointed) and F4/80-positive macrophages were greater in the cancerous liver of Sptbn1+/- old mouse than in WT mouse including hepatic sinusoids and perisinusoidal spaces of hepatic lobules.