Figure 6.

CK1ε antagonizes AES-mediated effect on Wnt and Notch signaling. (A) HEK293T cells were transfected with the indicated plasmids. Cell lysates were immunoprecipitated with IgG or anti-β-catenin agarose beads. The interaction of β-catenin with TCF4E was detected by immunoblotting. (B and C) HCT116 (B) and HT29 (C) cells were infected with the indicated lentivirus, respectively. Cell lysates were subjected to IP with IgG or anti-β-catenin agarose beads. Immunoblot analysis was performed to detect the interaction between β-catenin and TCF4E. (D-G) HCT116 (D and E) and HT29 (F and G) cells were infected with the indicated lentivirus. Then total RNA was extracted and real-time PCR was performed to detect the mRNA expression. Quantification of mRNA level was normalized to GAPDH. Values are shown as means ± SD (n = 3). *P < 0.05; Student's t test.