Figure 8.

CK1ε antagonizes the effect of AES on APC^min/+ colorectal tumor organoid growth and CRC metastasis in vivo. (A) Morphology of colorectal tumor organoids from B6-APC^min/+ mice infected with the indicated lentivirus. Right panel: graphical representation of quantitative data showed the relative size of organoid (n = 10). Scale bar, 200 μm. (B and C) HCT116 cells were infected with the indicated lentivirus and intrasplenically injected into nude mice, respectively. (B) Representative images of liver metastasis (n = 6). Right panel: graphical representation of quantitative data showed the relative rate of liver metastasis. (C) H&E staining. Scale bar, 200 μm. Values are shown as means ± SD. *P < 0.05; Student's t test.