Figure 5.

A HT diet does not affect TSPO expression as detected by [¹⁸F]DPA-714 PET imaging after ischemic injury. The mice were scanned for [¹⁸F]DPA-714 PET at days 7, 14, 21 and 30 post ischemia to assess TSPO expression within the infarct (white circle) and the contralateral striatum. The percentage of injected dose per millimetre (%ID/mL) was quantified within both regions. (A) Representative axial co-registered [¹⁸F]DPA-714-PET-CT images of a mouse fed with either control (upper row) or HT (lower row) diet after a 30 min tMCAo with comparable T₂w-infarct on day 1 post ischemia (white delineation). (B) Similar uptake was measured between both groups either within the infarct or contralateral striatum over time. (C) Infarct-to-contralateral ratios did not differ over time, indicative of constant chronic neuroinflammatory reaction in the infarcted hemisphere compared to the contralateral side (p > 0.05). Values represent mean ± sd. Control: n = 8, HT: n = 8. Statistical analysis was carried out with two-way RM ANOVA.