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. 2021 Feb 15;11(9):4030–4049. doi: 10.7150/thno.48110

Figure 6.

Figure 6

A HT diet induces a slight increase in TSPO expression at day 35 post ischemia. (A) Representative TSPO staining within the infarct and contralateral side obtained by immunohistochemistry at day 35 post ischemia from both control and HT-fed mice (white scale bar: 30 µm, black scale bar: 8 µm). For both experimental groups, immune cells and endothelial cells showed TSPO immunoreactivity. A HT diet slightly increased TSPO-related inflammatory level (n = 3/group, with 3 fields of view per animal). (B) Representative western blot for TSPO from brain protein extracts obtained at day 35 post ischemia (n = 6/group). A HT diet did not change TSPO protein levels in both the infarct (I) and contralateral (C) hemispheres at day 35 post ischemia. Values are normalized relative to GAPDH band. Statistical analysis was carried out with two-way ANOVA for multiple comparisons followed by Holm Sidak's post hoc test for multiple comparisons. Values represent mean ± sd. (*p < 0.05, ** p < 0.01, ***p < 0.005; * vs. contralateral, # vs. diet).