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. 2021 Feb 20;11(9):4262–4280. doi: 10.7150/thno.52309

Figure 3.

Figure 3

SAP-released Tβ4 promotes proliferation and migration of EPDCs. (A) The proliferated cells. The cells were treated for 3 days. The large boxes are magnification of the small boxes. Ki67 immunostaining. Scale bar = 100 µm. (B) Statistical result of the number of Ki67+ cells. (C) The number of total cells per field. n = 5. (D) Viability of the cells. The cells were treated for 3 days or 7 days. CCK-8 analysis. n = 5. *p < 0.05 versus control group; #p < 0.05 versus Tβ4 group; †p < 0.05 versus SAP group. (E) The cells migrated into the scratched region. The cells were incubated for 16 h after scratch. The dotted lines indicate the margin of the scratch. Scale bar = 100 µm. (F) Statistical result of the area of the migrated cells. n = 5. (G-I) Expression of Mylk, Rock1 and Vcl at 16 h after scratch. qRT-PCR analysis. *p < 0.05 and **p < 0.01 versus control group; #p < 0.05 versus Tβ4 group; †p < 0.05 and ††p < 0.01 versus SAP group. n = 4.