Figure 7.

Conditional deletion of SARM1 in neurons and astrocytes reduced the neuroinflammation through downregulation of NF-κB signaling after SCI. (A) Western blot analysis of the expression of NF-κB, IKB-α, p-JNK, JNK and c-Jun in the spinal cords of SARM1^f/f and SARM1^Nestin-CKO mice at different stages after SCI. (B-C) Quantitative analysis of the relative NF-κB (B) and IKB-α (C) levels as shown in (A) (two-way ANOVA (repeated measures) with Bonferroni's post-tests, n = 3 per group, normalized to sham group). (D-E) The volcano and heatmap of differentially expressed mRNAs of HSP family in the spinal cords of SARM1^f/f mice and SARM1^Nestin-CKO mice. (F) Western blot analysis of the expression of Hspa1a in the uninjured spinal cords or injured spinal cords at 3 d after SCI of SARM1^f/f and SARM1^Nestin-CKO mice. (G) Quantitative analysis of the relative Hspa1a levels as shown in (F) (two-tailed Student's t-test, n = 3 per group, normalized to sham group). Data were mean ± SEM. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001.