Figure 8.

Inhibition of HSP70 by apoptozole inhibited the recovery of behavior performance and neuronal regeneration in SARM1^Nestin-CKO mice after SCI. (A) Representative footprint images of SARM1^Nestin-CKO mice with or without apoptozole treatment at 3 d and 14 d after SCI. (B-C) Quantitative footprint analysis of stride length (B) and stride width (C) in footprint behavioral assay of SARM1^Nestin-CKO mice with or without apoptozole treatment at 3 d and 14 d after SCI (n = 6 per group). (D-E) Quantitative analysis of gross voluntary movement in open-field walking (D, n = 6 per group) and BMS scoring (E, n = 6 per group) of SARM1^Nestin-CKO mice with or without apoptozole treatment at 3 d and 14 d after SCI. (F) Double immunostaining analysis of NF (green) and MBP (red) in the spinal cords of SARM1^Nestin-CKO mice with or without apoptozole treatment at 14 d after SCI. (G-H) Quantitative analysis of the intensity of NF or MBP as shown in (F) (n = 6 per group, normalized to SARM1^Nestin-CKO mice without apoptozole treatment group). Dashed lines indicated the outline of the injury sites. Images of selected regions (rectangles) in (F) were shown at higher magnification. Scale bars, 1 cm (A), 20 µm (F). Data were mean ± SEM. Two-tailed Student's t-test, ^*P < 0.05, ^**P < 0.01, ^***P < 0.001.