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. 2021 Feb 25;11(9):4298–4315. doi: 10.7150/thno.51342

Figure 6.

Figure 6

METTL3 promotes CRC cell invasion and migration via the circ1662-YAP1-SMAD3 axis. (A, B) Rescues of transwell assays when HCT116 and SW480 cells were transfected METTL3-overexpressed vector and subsequent circ1662 siRNA. (C, D) The efficiency of METTL3 and circ1662 when constructing double-lentivirus transfected HCT116 using qPCR, GAPDH is the negative control. (E) The expression of SMAD3 in double-lentivirus transfected HCT116 using qPCR, GAPDH is the negative control. (F) The number of lung metastasis nodules from Bala/c mice injected stable double-lentivirus HCT116 via tail vein (n = 3 each group). (K) HE staining, ISH staining of circ1662 and IHC staining of METTL3, YAP1, SMAD3 and E-cadherin in lung metastases from Bala/c mice injected stable double-lentivirus HCT116 via tail vein. GAPDH is used as an internal reference for performing qPCR. Statistical significance was calculated by Student's t test, *P < 0.05, 0.001 < ** P < 0.01, *** P < 0.001, Mean ± SEM.