Figure 6.

Latrunculin A and C3 inhibit osteogenic gene expression and mineralization on MC but not NX-MC. QPCR of primary hMSCs cultured on NX-MC or MC for 0 or 3 d in osteogenic differentiation medium untreated, treated with 0.5 × 10⁻⁶m of LA, or treated with 3 µg mL⁻¹ of C3 for A) ALP, B) COL1A1, and C) RUNX2. D) Quantitative Alizarin red analysis of empty scaffolds (Empty) or hMSCs cultured on NX-MC or MC for 0 (D0) or 14 d (D14) in osteogenic differentiation medium untreated, treated with 0.5 × 10⁻⁶m of LA, or treated with 3 µg mL⁻¹ of C3 (n = 5). Bars represent means, errors bars represent SE. Significant posthoc comparisons following ANOVA indicated with p-values.