Figure 7.

Inhibition of Rho GTPase or F-actin polymerization reduces active β-catenin with a compensatory increase in p-Smad1/5 and BMP2 expression. Western blot of primary hMSCs cultured on NX-MC or MC for 0 or 3 d in osteogenic differentiation medium untreated, treated with 0.5 µM of LA, or treated with 3 µg mL⁻¹ of C3 for A) Runx2, OPG, non-p-β-catenin, and GAPDH or D) p-Smad1/5 and Smad5. Densitometric quantification of western blot analyses demonstrating relative protein amounts of B) Runx2 to GAPDH, C) non-p-β-catenin to GAPDH, or E) p-Smad1/5 to Smad5. QPCR of primary hMSCs cultured on NX-MC or MC for 0 or 3 d in osteogenic differentiation medium untreated, treated with 0.5 µM of LA, or treated with 3 µg mL⁻¹ of C3 for F) BMP2, G) BMP4, and H) BMP7. Bars represent means, errors bars represent SE. Significant posthoc comparisons following ANOVA indicated with p-values.