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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: Nat Microbiol. 2020 Oct 19;5(12):1576–1587. doi: 10.1038/s41564-020-00795-7

Fig. 3. Activation of the GSDMD-dependent pyroptosis pathway induces passive release of SQSTM1.

Fig. 3.

(a-c) Analysis of cytotoxicity (a), HMGB1 release (b), and SQSTM1 release (c) in the indicated LPS-primed BMDMs following LPS electroporation (LPSe) or E. coli (MOI = 25) infection for 24 h (n = 5 well/group; two-tailed t test, versus wild type group). (d) Survival of the indicated mice primed with poly (I:C) (10 mg/kg, i.p.) and then challenged 6 h later with LPS (2 mg/kg, i.p.; n = 10 mice/group; Log-rank test). (e-h) In parallel, the serum level of IL1B (e), HMGB1 (f), LDH (g), and SQSTM1 (h) were assayed (n = 5 mice/group; two-tailed t test, versus wild type group). Data in (a-c) and (e-h) are presented as mean ± SD. Data in (d-h) are from two independent experiments. Data in (a-c) are from three independent experiments.