Skip to main content
. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: Nat Microbiol. 2020 Oct 19;5(12):1576–1587. doi: 10.1038/s41564-020-00795-7

Extended Data Fig. 2. The activation of the GSDMD-dependent pyroptosis pathway induces SQSTM1 release.

Extended Data Fig. 2.

(a) Analysis of cytotoxicity, HMGB1 release, and SQSTM1 release in the indicated poly (I:C)-primed BMDMs following LPS electroporation (LPSe) or E. coli (MOI = 25) infection for 24 h (n = 5 well/group; two-tailed t test, versus wild type group). (b) Analysis of cytotoxicity, HMGB1 release, and SQSTM1 release in the indicated LPS-primed BMDMs following ATP (5 mM, 3 h) or nigericin (10 μM, 3 h) treatment (n = 5 well/group; two-tailed t test, versus wild type group). (c) Analysis of cytotoxicity, HMGB1 release, and SQSTM1 release in the indicated BMDMs following Y. pestis (MOI = 25) infection or LPS5Z7 (LPS [50 ng/ml] + 5z7 [400 nM]) (n = 5 well/group; two-tailed t test, versus wild type group). Data in (a-c) are presented as mean ± SD from two independent experiments.