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. 2021 Mar 4;11(10):4743–4758. doi: 10.7150/thno.52102

Figure 4.

Figure 4

Effects of LY2157299 on liver regeneration. (A) Experiment design: Rats were treated with DEN for 12 weeks and then orally administered 150 mg/kg LY2157299 twice per day for 7 days before sacrifice. The rats were sacrificed on days 2 and 7 after Step I (n = 5). NC rats were administered 1% CMC intragastrically. (B) HNF4α/TGFβR1 co-staining of day 2 FLR tissue sections demonstrating that TGFβR1 was mainly located on the membrane of hepatocytes (scale bars, 50 µm). (C) BW and FLR/BW of rats treated with LY2157299 or CMC after surgery (n = 5, two-tailed ANOVA). (D) qRT-PCR analysis of TGFβ1 mRNA demonstrating upregulation in the LY2157299-treated group on days 2 and 7 post-surgery (n = 5, two-tailed ANOVA). (E) Hepatic protein expression (normalized to GAPDH) of TGFβ1, pSMAD2, cyclin E1, and CKD2 in the LY2157299 and CMC groups. (F) Representative images of liver tissue stained with Ki67, EdU, and PSR (scale bars, 50 µm). The percentage of Ki67+ and EdU+ cells and the PSR-stained area were analyzed separately (n = 4, two-tailed ANOVA). No significant difference in PSR-stained area was observed between the two groups.