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FPLC purification of fermentation-produced miRNA agents and HPLC determination of recombinant ncRNAs purities. (A) FPLC-UV traces during the purification of htRNALeu/miR-34a-5p, htRNALeu/miR-124-3p, and control htRNALeu. The insert shows urea-PAGE analysis of corresponding target RNA fractions #1 to 6. (B) The purities of final htRNALeu/miR-34a-5p, htRNALeu/miR-124-3p, and control htRNALeu molecules were determined by HPLC analyses, which are all over 99% pure.
