Figure 1.

Aurka was deleted in CD19⁺ B cells. (A) The mRNA levels of Aurka were examined in sorted RCB, IMB, pre/pro B and pre-pro B cells. *, P < 0.05, n.s., not significant. (B) The mRNA levels of Aurka were examined in sorted CD4⁺ T, CD8⁺ T and CD19⁺ B cells. *, P < 0.05, n.s., not significant. (C) The mRNA levels of Aurka were examined in sorted megakaryocytes and macrophages. (A-C) Data shown is mean ± SD of one of three independent experiments (n = 3 mice/group) with similar results. Significance was calculated using an unpaired Student's t-test. *, P < 0.05. (D) Aurora-A expression was examined in sorted CD19⁺ B cells from either Aurka^f/f mice (n = 2) or Aurka^f/f;Cd19^Cre/+ mice (n = 2) by Western blotting. The data shown is representative of one of two independent experiments.