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. 2021 Mar 4;11(10):4655–4671. doi: 10.7150/thno.49007

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B cell development was impaired in the spleen. (A) Approximately 1 × 10⁵ nucleated cells in the spleens of mice with different genotypes were analyzed. The distribution of various B-cell populations in the spleen of mice with different genotypes was assessed by flow cytometry. The data shown is representative of one of two independent experiments. (B) The dot graph shows the percentage of CD19⁺ B cells gated on CD45⁺ cells in the spleens removed from the Aurka^f/f mice or the Aurka^f/f;Cd19^Cre/+ mice. Data shown are mean ± SD of one of two independent experiments (n = 9 mice/group) with similar results. P-value was calculated using an unpaired Student's t-test. *, P < 0.05. (C) The dot graph shows the percentage of various B-cell populations in the total B cells of the spleens removed from the Aurka^f/f mice or the Aurka^f/f;Cd19^Cre/+ mice. *, P < 0.05. (D) The dot graph shows the total number of indicated B cells in spleens. The total number of indicated cells was calculated with the following formula: the number of indicated B cells/the number of gated single cells × the total nucleated cells recovered from the spleen. *, P < 0.05. (E) CD19⁺ B cells (1 × 10⁵) in the spleens removed from the Aurka^f/f mice or the Aurka^f/f;Cd19^Cre/+ mice were analyzed. The dot graph shows the total number of CD27⁺ memory B cells in these CD19⁺ B cells. *, P < 0.05. (C-E) Data shown are mean ± SD of one of two independent experiments (n = 6 mice/group) with similar results. Significance was calculated using an unpaired Student's t-test. (F) The dot graph shows the spleen weight of either the Aurka^f/f mice or the Aurka^f/f;Cd19^Cre/+ mice. The data shown are representative of one of two independent experiments (n = 7 mice/group) with similar results. Significance was calculated using an unpaired Student's t-test. (G) The dot graph shows the total number of nucleated cells in the spleens of either the Aurka^f/f mice or the Aurka^f/f;Cd19^Cre/+ mice. The data shown are representative of one of two independent experiments (n = 6 mice/group) with similar results. Significance was calculated using an unpaired Student's t-test.