Figure 6.

Aurka deficiency in CD19⁺ B cells induced TPO production. (A) The concentrations of TPO in the plasma were examined by ELISA. * P < 0.05. (B) Frozen livers were weighed, homogenized, and followed by sonication. After centrifugation, the supernatant was collected and subjected to ELISA to examine the concentrations of TPO. The concentrations of TPO in the liver were calculated following the formula: (concentration × sample volume × diluent factor)/weight. * P < 0.05. (C) Frozen spleen were weighed, homogenized, and followed by sonication. After centrifugation, the supernatant was collected and subjected to ELISA. The concentrations of TPO in the spleen were examined by ELISA, and calculated following the formula: (concentration × sample volume × diluent factor)/weight. * P < 0.05. (A-C) The dot graphs show one of two independent experiments (n = 6 mice/group) with similar results. P-value was calculated using an unpaired Student's t-test. (D) Real-time RT-PCR to quantify the Mpl mRNA levels in sorted CD41⁺Mpl⁺ megakaryocytes from the Aurka^f/f or Aurka^f/f;Cd19^Cre/+ mice. *P < 0.05. (E) Real-time RT-PCR to quantify Gata1 mRNA levels in the sorted CD41⁺Mpl⁺ megakaryocytes from the Aurka^f/f or Aurka^f/f;Cd19^Cre/+ mice. * P < 0.05. (D-E) The graphs show one of two independent experiments (n = 3 mice/group) with similar results. Significance was calculated using an unpaired Student's t-test. (F) The graph shows the total number of platelets in the peripheral blood of the indicated mice treated with either control diluent or mTPO. The data shows one of two independent experiments (n = 5 mice/group) with similar results. Significance was calculated using two way ANOVA followed by Tukey's multiple comparisons test. *, P < 0.05.