Figure 2.

GO analysis of differentially expressed SNAP29-interacting proteins, using Database for Annotation, Visualization, and Integrated Discovery (DAVID). A. The most significantly enriched Kyoto Encyclopedia of KEGG pathway of the differentially expressed proteins was cytoplasm transportation. The synaptic vesicle cycle and glutamatergic transmission processes are particularly noteworthy, given the use of neurons. B. Differentially expressed SNAP29-interacting proteins mainly located in the cytoplasm or associated with vesicles. C. The identified SNAP29 interacting differentially expressed proteins in PPI network were involved in the splicesome, RNA transport, oocyte meiosis, phagosome, glyxylate and dicarboxylate metabolism, gap junction, protein export and synaptic vesicle cycle, which suggests that SNAP29-associated synaptic function may be of great importance in the OGD and/or reperfusion process.