Table 2.
The list of reagents and consumables used in the current study
| Reagent | Brand | Cat. |
|---|---|---|
| RIPA | Cell Signal Technology | 9806S |
| Revert Aid First Strand cDNA Synthesis Kit | ThermoFisher Scientific | K1622 |
| PowerUp™ SYBR™ Green Master Mix | ThermoFisher Scientific | A25742 |
| DAPI | SouthernBiotech | 0100-29 |
| Poly-vinylidene difluoride membranes | Healthcare Life science | 0.2 μm PVDF |
| Pierce™ BCA Protein Assay Kit | ThermoFisher Scientific | 23225 |
| No glucose DMEM | ThermoFisher Scientific | 11966025 |
| CCK-8 | Beyotime Biotechnology | C0038 |
| Silicone thread embolism | RWD Life Science | 970-00091-00 |
| Lactate dehydrogenase assay kit | abcam | Ab102526 |
| Bicuculline | Sigma-Aldrich | S2694-5 MG |
| D(-)-2-Amino-5-phosphonopentanoic acid | MedChemEpress | HY-100714A |
| TTX | Sigma-Aldrich | A3109 |
| Thick‐walled boro‐silicate glass | VWR Scientific | N/A |
| DMEM, high glucose, Pyruvate | ThermoFisher Scientific | 11995065 |
| NeurobasalTM-A Medium | ThermoFisher Scientific | 11888022 |
| B-27TM Supplement (50×), serum free | ThermoFisher Scientific | 17504044 |
| Calcium chloride | Sigma-Aldrich | C4901-100 G |
| Magnesium chloride | Sigma-Aldrich | M8266-100 G |
| D(-)-2-Amino-5-phosphonopentanoic acid | Sigma-Aldrich | A8054-1 MG |
| HBSS-Hank's, calcium, magnesium, no phenol red | ThermoFisher Scientific | 14025076 |
| Trypsin-EDTA (0.25%), phenol red | ThermoFisher Scientific | 25200056 |
| MG132 | MedChemExpress | HY-13259 |
| PhosSTOP EASYpack; Phosphatase Inhibitor Cocktail Tablets Supplied in Foil Blister Packs | Roche | 04906845001 |
| Protease Inhibitor Cocktail | Sigma-Aldrich | P8340-1 ml |
| Glycine | Sigma-Aldrich | V900114-500 G |
| Sodium dodecyl sulfate | Sigma-Aldrich | L5750-1 KG |
| Acrylamide | Sigma-Aldrich | V900845-1 KG |
| Tris base | Sigma-Aldrich | 10708976001-1 KG |
| Sodium chloride | Gong Ke Ji You Xian Co.,Ltd | N/A |
| Glutaraldehyde solution (50%) | Sinopharm Chemical Reagent Co.,Ltd | 30092595-250 ML |
| N,N,N′,N′-Tetramethylethylenediamine | Sigma-Aldrich | T7024-25 ML |
| Ammonium persulfate | Sigma-Aldrich | A9154-100 G |
| PBS Buffer powder | Zhong Shan Jin Qiao Co.,Ltd | ZLI-9061 |
| Non-fat milk powder | Pu Li Lai Co.,Ltd | P1622 |
| TWEEN® 20 | Sigma-Aldrich | V900548-500 ML |
| Paraformaldehyde | Sigma-Aldrich | 16005-1 KG-R |
| Adenosine 5′-triphosphate disodium salt solution | Sigma-Aldrich | A6559-25 UMO |
| Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid | Sigma-Aldrich | E0396-10 G |
| Potassium chloride | Bei Jing Shi Ji | N/A |
| 6 Well Cell Culture Plate | Sigma-Aldrich | 3516 |
| 24 Well Cell Culture Plate | Sigma-Aldrich | 3524 |
| 96 Well Cell Culture Plate | Sigma-Aldrich | 3599 |
| Triton X-100 | Zhong Shan Jin Qiao Co.,Ltd | ZLI-9308 |
| Fetal Bovine Serum, qualified, heat inactivated, Australia | ThermoFisher Scientific | 10100147 |
| Fetal Equine Serum | Solarbio Life Science | S9050-200 |
| Poly-D-lysine hydrobromide | Sigma-Aldrich | P1149-500 MG |
| Microscope Cover Glass | ThermoFisher Scientific | 12-545-80 12CIR.-1 |
| Cell Strainer 70 μm Nylon | Corning | 352350 |
| 15 ml Polypropylene Conical Centrifuge Tube | R&D | KG2611 |
| 50 ml Polypropylene Conical Centrifuge Tube | R&D | KG2811 |
| PageRuler™ Prestained Protein Ladder | ThermoFisher Scientific | 26616 |