Figure 3.

FOSL2 promotes CAF angiogenesis in a VEGF-independent manner. (A) VEGFA levels were detected by western blotting and normalized to β-actin in the indicated cells. (B) ELISA was used to determine the VEGFA concentrations in the supernatants of the indicated cells. (C, E) Representative images of the formation of HUVEC tubes following incubation with CM derived from CAFs or NFs treated with VEGF Ab; the average branch number was calculated. (D, F) Representative images of the spheroid spouting of HUVECs following incubation with CM collected from CAFs or NFs treated with VEGF Ab; the cumulative sprout length was calculated (scale bar, 100 μm).